Determination of ibuprofen and flurbiprofen in pharmaceuticals by capillary zone electrophoresis

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 41; no. 4; pp. 1463 - 1467
• Main Authors: HAMOUDOVA, Rafifa, POSPISILOVA, Marie
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 16.06.2006; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Anti-Inflammatory Agents, Non-Steroidal - analysis; Biological and medical sciences; Capillary electrophoresis; Electrophoresis, Capillary - methods; Flurbiprofen; Flurbiprofen - analysis; Fundamental and applied biological sciences. Psychology; General pharmacology; Ibuprofen; Ibuprofen - analysis; Medical sciences; Pharmaceutical analysis; Pharmaceutical Preparations - analysis; Pharmacology. Drug treatments; Reproducibility of Results; Stereoisomerism; Tablets; Pharmaceutical analysis; Capillary electrophoresis; Ibuprofen; Flurbiprofen; Non steroidal antiinflammatory agent; Prostaglandin-endoperoxide synthase; Zone electrophoresis; Enzyme; Enzyme inhibitor; Arylpropionic acid derivatives; Oxidoreductases; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2006.03.024

Capillary zone electrophoresis with spectrophotometric detection was used for the determination of ibuprofen (IB) and flurbiprofen (FL) in pharmaceuticals. The separation was carried out in a fused silica capillary (60 cm × 100 μm i.d. effective length 45 cm) at 30 kV with UV detection at 232 nm. The optimized background electrolyte was 20 mM N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) with 20 mM imidazole and 10 mM α-cyclodextrin of pH 7.3. 2-Naphthoxyacetic acid was used as internal standard. A single analysis took less than 5 min. Rectilinear calibration ranges were 2–500 mg l −1 for IB and 1–60 mg l −1 for FL. The relative standard deviations (R.S.D.) values ( n = 6) were 1.53% for IB and 1.29% for FL (for 200 mg l −1 IB and 10 mg l −1 FL). This validated method has been successfully applied for the routine analysis of 10 commercially available pharmaceutical preparations (syrup, tablets, cream and gel).