The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

• Published in: Plants (Basel) Vol. 13; no. 9; p. 1264
• Main Authors: Park, Hye-Wan, Sevilleno, Samantha Serafin, Ha, My Khanh Tran Thi, Cabahug-Braza, Raisa Aone, Yi, Ji-Hun, Lim, Ki-Byung, Cho, Wonwoo, Hwang, Yoon-Jung
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.05.2024
• Subjects: Cell cycle; Chromosomes; Crop development; Cultivars; Cytogenetics; Females; FISH; Fluorescence; Fluorescence in situ hybridization; Gene sequencing; Genetic diversity; Hybridization; Hybrids; Identification methods; marker-assisted breeding; Nucleotide sequence; Plant breeding; Plant reproduction; rDNA; Reproductive system; Ribosomal DNA; Veronica; FISH; rDNA; cytogenetics; marker-assisted breeding; Veronica
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13091264

Fluorescence in situ hybridization (FISH), a molecular cytogenetic technique that enables the visualization and identification of specific DNA sequences within chromosomes, has emerged as a pivotal tool in plant breeding programs, particularly in the case of species. , a genus with a complex reproductive system, often poses challenges in accurately identifying hybrids because of its tendency to hybridize, which leads to intricate genetic variation. This study focused on the use of FISH as a prescreening method to identify true hybrids in breeding programs. FISH analysis was first performed on the parents to identify their 45S and 5S rDNA signals, along with their respective chromosome numbers. The signals were then compared with those of the twenty progenies with reference to their supposed parents. Five true hybrids, seven self-pollinated progenies, and eight false hybrids were identified through FISH. The findings highlight the significance of FISH as a screening method that contributes significantly to the efficiency of breeding programs by ensuring the preservation of desired genetic traits and minimizing the inadvertent inclusion of misidentified hybrids. To conclude, this study underscores the vital role of FISH in enhancing the precision and success of breeding programs and opens new avenues for improved breeding strategies and crop development.