Hepcidin generated by hepatoma cells inhibits iron export from co-cultured THP1 monocytes

• Published in: Journal of hepatology Vol. 44; no. 6; pp. 1125 - 1131
• Main Authors: Andriopoulos, Bill, Pantopoulos, Kostas
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 01.06.2006; Elsevier
• Subjects: Anemia of chronic disease; Anemias. Hemoglobinopathies; Antimicrobial Cationic Peptides - biosynthesis; Antimicrobial Cationic Peptides - genetics; Biological and medical sciences; Biological Transport; Carcinoma, Hepatocellular; Cell Line, Tumor; Coculture Techniques; Diseases of red blood cells; DNA, Complementary - genetics; Ferroportin; Gastroenterology. Liver. Pancreas. Abdomen; Hematologic and hematopoietic diseases; Hemochromatosis; Hemojuvelin; Hepatocytes - drug effects; Hepatocytes - metabolism; Hepcidins; HFE; Humans; Interleukin-6 - pharmacology; Iron - metabolism; Liver - cytology; Liver - drug effects; Liver - metabolism; Liver Neoplasms; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Macrophages - metabolism; Medical sciences; Metabolic diseases; Metals (hemochromatosis...); Monocytes - metabolism; Other metabolic disorders; Transfection; Tumors; Ferroportin; Hemochromatosis; Hemojuvelin; HFE; Anemia of chronic disease; Cell culture; Monocyte; Anemia; Chronic disease; Hepatic disease; Metabolic diseases; Hepatocellular carcinoma; Hemopathy; Iron; Malignant tumor; Enzymopathy; Gastroenterology; Digestive diseases
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/j.jhep.2005.10.025

The antimicrobial peptide hepcidin is generated in the liver and released into the circulation in response to iron, oxygen and inflammatory signals. Hepcidin serves as a hormonal regulator of duodenal iron absorption and iron trafficking in the reticuloendothelial system. The aim of this study is to explore the effects of this regulatory peptide in macrophage iron metabolism. Hepcidin-mediated iron efflux and parameters of cellular iron homeostasis were studied in THP1 monocytic cells co-cultured with hepcidin-producing hepatic cells. Stimulation of hepcidin expression in Huh7 cells with interleukin-6 promoted a significant ∼30% decrease in 59Fe efflux from THP1 cells, previously loaded with 59Fe-transferrin. Similar results were obtained with HepG2 cells transfected with a hepcidin cDNA. Importantly, hepcidin expression from Huh7 cells elicited a decrease in the levels of the iron-sensitive post-transcriptional regulator IRP2 in THP1 cells, accompanied by de novo synthesis of the iron storage protein ferritin. Physiologically generated hepcidin inhibits iron efflux and promotes iron accumulation in monocytic cells, mimicking a pathophysiological response commonly observed in the anemia of inflammation. Our results highlight the crucial role of hepcidin in the control of macrophage iron homeostasis.