Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration

We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at...

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Bibliographic Details
Published inBiomedical optics express Vol. 6; no. 6; pp. 2056 - 2066
Main Authors Trivedi, Vikas, Truong, Thai V, Trinh, Le A, Holland, Daniel B, Liebling, Michael, Fraser, Scott E
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 01.06.2015
Subjects
(170.3010) Image reconstruction techniques
(190.0190) Nonlinear optics
(170.1420) Biology
(180.0180) Microscopy
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Summary:We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at 70 frames per second, and the individual optical sections were assembled into a full 4D reconstruction of the beating heart using an optimized retrospective image registration algorithm. This imaging and reconstruction platform permitted us to visualize protein expression patterns at endogenous concentrations in zebrafish gene trap lines.
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ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.6.002056