Immuno-electron-cytochemical localization of the somatostatin cells in the human antral mucosa

Immuno-cytochemical methods were used to identify, in light and electron microscopy, the somatostatin-containing cells of the human antral mucosa. By means of immunoperoxidase and immunofluorescence methods sequentially applied on the same section, it was shown that the somatostatin cells are distin...

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Bibliographic Details
Published inThe journal of histochemistry and cytochemistry Vol. 25; no. 10; pp. 1111 - 1118
Main Authors Canese, MG, Bussolati, G
Format Journal Article
LanguageEnglish
Published Los Angeles, CA Histochemical Soc 01.10.1977
SAGE Publications
Subjects
Fluorescent Antibody Technique
Gastric Mucosa - cytology
Gastric Mucosa - ultrastructure
Histocytochemistry
Humans
Microscopy, Electron
Peroxidases - immunology
Pyloric Antrum - cytology
Somatostatin - biosynthesis
Staining and Labeling
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Summary:Immuno-cytochemical methods were used to identify, in light and electron microscopy, the somatostatin-containing cells of the human antral mucosa. By means of immunoperoxidase and immunofluorescence methods sequentially applied on the same section, it was shown that the somatostatin cells are distinct from the gastrin cell population; these two endocrine cell types are often closely related. On ultrathin sections from aldehyde-fixed. Epon-araldite embedded tissues, the site of storage of somatostatin was localized with the peroxidaseantiperoxidase complexes technique, after removal of the resin by means of sodium ethoxide. This procedure represents a new technical approach to the use of electron-cytochemical techniques. The results indicate that somatostatin, a growth hormone release inhibiting factor, is localized in the endocrine granules of the D cells.
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ISSN:0022-1554
1551-5044
DOI:10.1177/25.10.72094