Stem Cell Oriented Exosomes Regulate Cell Proliferation in Hepatoma Carcinoma

Reprogrammed hepatic stem cells can generate a variety of extracellular vesicular particles including exosomes with similar therapeutic potential. However, their functional application in cancer therapy is restricted due to a poor understanding of their physical properties, anti-proliferative effect...

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Published inBiotechnology and bioprocess engineering Vol. 28; no. 2; pp. 263 - 273
Main Authors Karima, Gul, Shin, Kyusoon, Jeong, Jaemin, Choi, Dongho, Hwang, Kyung-Gyun, Hong, Jong Wook
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Biotechnology and Bioengineering 01.04.2023
Springer Nature B.V
한국생물공학회
Subjects
Antiproliferatives
B-cell lymphoma
Biotechnology
Cancer
cancer therapy
carcinoma
Caspase-3
CD63 antigen
CD81 antigen
Cell growth
Cell proliferation
Chemistry
Chemistry and Materials Science
Exosomes
Hepatoma
Industrial and Production Engineering
Liver
Liver cancer
Lymphocytes B
Lymphoma
Mitochondria
Physical properties
Research Paper
Size distribution
Stem cells
생물공학
suppression
stem cells
cell proliferation
exosome
hepatocellular carcinoma
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Summary:Reprogrammed hepatic stem cells can generate a variety of extracellular vesicular particles including exosomes with similar therapeutic potential. However, their functional application in cancer therapy is restricted due to a poor understanding of their physical properties, anti-proliferative effects, and the underlying mechanism. Here, we explore reprogrammed stem cells that can release vesicular particles with physical properties of exosomes that can exert anti-proliferative effects by regulating cell proliferation-related genes. We obtain vesicular particles with properties of exosomes expressing CD81 and CD63 with the size distribution peak observed at 155 nm. We analyze that as compared to non-treated conditions, vesicular particles have reduced cell proliferation of Hep G2 by 93.6% after 72 h. Furthermore, we also identify that B-cell lymphoma 2 has been reduced by 89.64% and activation of caspase-3 has occurred in treated conditions via the mitochondrial pathway. Therefore, our results may highlight the potential of exosomes from hepatic stem cells to play a vital role in treating liver pathologies.
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ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-022-0238-y