Cellular Immune Responses in Humans Induced by Two Serogroup B Meningococcal Outer Membrane Vesicle Vaccines Given Separately and in Combination

• Published in: Clinical and vaccine immunology Vol. 23; no. 4; pp. 353 - 362
• Main Authors: Oftung, Fredrik, Korsvold, Gro Ellen, Aase, Audun, Næss, Lisbeth M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2016
• Subjects: Adult; CD4-Positive T-Lymphocytes - immunology; Cell Proliferation; Cytokines - secretion; Extracellular Vesicles - immunology; Humans; Immunity, Cellular; Immunity, Heterologous; Immunization Schedule; Meningococcal Vaccines - administration & dosage; Meningococcal Vaccines - immunology; Neisseria meningitidis, Serogroup B - immunology; Vaccines
• ISSN: 1556-6811; 1556-679X
• DOI: 10.1128/CVI.00666-15

MenBvac and MeNZB are safe and efficacious outer membrane vesicle (OMV) vaccines against serogroup B meningococcal disease. Antibody responses have previously been investigated in a clinical trial with these two OMV vaccines given separately (25 μg/dose) or in combination (12.5 and 12.5 μg/dose) in three doses administered at 6-week intervals. Here, we report the results from analyzing cellular immune responses against MenBvac and MeNZB OMVs in terms of antigen-specific CD4(+)T cell proliferation and secretion of cytokines. The proliferative CD4(+)T cell responses to the combined vaccine were of the same magnitude as the homologous responses observed for each individual vaccine. The results also showed cross-reactivity in the sense that both vaccine groups receiving separate vaccines responded to both homologous and heterologous OMV antigen when assayed for antigen-specific cellular proliferation. In addition, a multiplex bead array assay was used to analyze the presence of Th1 and Th2 cytokines in cell culture supernatants. The results showed that gamma interferon, interleukin-4 (IL-4), and IL-10 responses could be detected as a result of vaccination with both the MenBvac and the MeNZB vaccines given separately, as well as when given in combination. With respect to cross-reactivity, the cytokine results paralleled the observations made for proliferation. In conclusion, the results demonstrate that cross-reactive cellular immune responses involving both Th1 and Th2 cytokines can be induced to the same extent by different tailor-made OMV vaccines given either separately or in combination with half the dose of each vaccine.