FtsQ interaction mutants: a way to identify new antibacterial targets

• Published in: New biotechnology Vol. 27; no. 6; pp. 870 - 881
• Main Authors: Grenga, L., Guglielmi, G., Melino, S., Ghelardini, P., Paolozzi, L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.12.2010
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Bacterial Infections - drug therapy; Cell division; Escherichia coli; Escherichia coli - cytology; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Genetic Complementation Test; Humans; Membrane Proteins - genetics; Membrane Proteins - metabolism; Point Mutation
• ISSN: 1871-6784; 1876-4347
• DOI: 10.1016/j.nbt.2010.05.002

FtsQ is a highly conserved component of the divisome that plays a central role in the assembly of early and late cell division proteins. The biological activity of this protein is still largely unknown, but its ability to interact with many components of the divisome was described by both two-hybrid assays and co-immunoprecipitation experiments. This paper describes the behaviour of ftsQ point mutants, created by random mutagenesis without regard to their phenotype, in which FtsQ is impaired in its ability to interact with its Escherichia coli division partners. Our results allow the identification of FtsQ residues involved in the interaction with other partner proteins and the determination of the biological significance of these interactions. The knowledge derived by this study could constitute not only the basis for understanding how these proteins assemble in the divisome, but also a starting point for the design of new antibacterial drugs that disrupt the bacterial division machinery.