Nanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles

• Published in: Materials Science & Engineering C Vol. 109; p. 110428
• Main Authors: Deák, Róbert, Mihály, Judith, Szigyártó, Imola Cs, Beke-Somfai, Tamás, Turiák, Lilla, Drahos, László, Wacha, András, Bóta, Attila, Varga, Zoltán
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2020; Elsevier BV
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Cell membranes; Dipalmitoyl phosphatidylcholine; Erythrocytes; Ghost; Ghosts; Lipid Bilayers - chemistry; Lipids; Lysophospholipids - chemistry; Materials science; Membrane proteins; Membrane vesicles; Membranes - chemistry; Micelles; Nanoerythrosome; Phospholipids; Protein - lipid interactions; Protein Folding; Protein network; Protein scaffolding; Proteins; Scaffolding; Spectrin; Stiffness; Structural proteins; Protein scaffolding; Nanoerythrosome; Protein network; Ghost; Protein - lipid interactions
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2019.110428

A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced. [Display omitted] •The synthesis of a wide size- and functional variety of nanoerythrosomes from human erythrocyte ghost membranes, in the size range between 20 and 160 nm•Tailoring of nanoerythosomes by the variation of the type and ratio of extra added lipids•A peculiar polyhedral nanoerythrosomes with enhanced rigidity can be constructed•A polygonal protein scaffolding of nanoerythrosomes can be reversibly disarrayed and also reconstructed by heating-cooling cycles•Lysophospholipid acts as detergent and disrupts the protein pattern of nanoerythrosomes