Isolation and Characterization of a Distinct Influenza A Virus from Egyptian Bats

• Published in: Journal of virology Vol. 93; no. 2
• Main Authors: Kandeil, Ahmed, Gomaa, Mokhtar R., Shehata, Mahmoud M., El Taweel, Ahmed N., Mahmoud, Sara H., Bagato, Ola, Moatasim, Yassmin, Kutkat, Omnia, Kayed, Ahmed S., Dawson, Patrick, Qiu, Xueting, Bahl, Justin, Webby, Richard J., Karesh, William B., Kayali, Ghazi, Ali, Mohamed A.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 15.01.2019
• Subjects: Animals; Antibodies, Viral - metabolism; Chickens; Chiroptera - virology; Dogs; Egypt; Genetic Diversity and Evolution; Influenza A virus - classification; Influenza A virus - genetics; Influenza A virus - isolation & purification; Lung - virology; Madin Darby Canine Kidney Cells; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - virology; Phylogeny; RNA, Viral - genetics; Sequence Analysis, RNA - methods; Egypt; influenza; veterinary epidemiology; public health; surveillance studies
• ISSN: 0022-538X; 1098-5514; 1098-5514
• DOI: 10.1128/JVI.01059-18

Through surveillance, we isolated and characterized an influenza A virus from Egyptian fruit bats. This virus had an affinity to avian-like receptors but was also able to infect mice. Our findings indicate that bats may harbor a diversity of influenza A viruses. Such viruses may have the potential to cross the species barrier to infect other species, including domestic birds, mammals, and, possibly, humans. Recently, two genetically distinct influenza viruses were detected in bats in Guatemala and Peru. We conducted influenza A virus surveillance among four bat species in Egypt. Out of 1,202 swab specimens, 105 were positive by real-time PCR. A virus was successfully isolated in eggs and propagated in MDCK cells in the presence of N -tosyl- l -phenylalanine chloromethyl ketone-treated trypsin. Genomic analysis revealed that the virus was phylogenetically distinct from all other influenza A viruses. Analysis of the hemagglutinin gene suggested a common ancestry with other H9 viruses, and the virus showed a low level of cross-reactivity with serum raised against H9N2 viruses. Bats were seropositive for the isolated viruses. The virus replicated in the lungs of experimentally infected mice. While it is genetically distinct, this virus shares several avian influenza virus characteristics suggesting a more recent avian host origin. IMPORTANCE Through surveillance, we isolated and characterized an influenza A virus from Egyptian fruit bats. This virus had an affinity to avian-like receptors but was also able to infect mice. Our findings indicate that bats may harbor a diversity of influenza A viruses. Such viruses may have the potential to cross the species barrier to infect other species, including domestic birds, mammals, and, possibly, humans.