Identification of a Conserved, Orphan G Protein-Coupled Receptor Required for Efficient Pathogen Clearance in Caenorhabditis elegans

• Published in: Infection and immunity Vol. 87; no. 4
• Main Authors: Anderson, Alexandra, Chew, Yee Lian, Schafer, William, McMullan, Rachel
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2019
• Subjects: Actinobacteria - physiology; Animals; Caenorhabditis elegans - genetics; Caenorhabditis elegans - immunology; Caenorhabditis elegans - microbiology; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - immunology; Host Response and Inflammation; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - immunology; G protein-coupled receptor; Caenorhabditis elegans; Microbacterium nematophilum; pathogen clearance
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00034-19

G protein-coupled receptors contribute to host defense across the animal kingdom, transducing many signals involved in both vertebrate and invertebrate immune responses. While it has become well established that the nematode worm triggers innate immune responses following infection with numerous bacterial, fungal, and viral pathogens, the mechanisms by which recognizes these pathogens have remained somewhat more elusive. G protein-coupled receptors have been implicated in recognizing pathogen-associated damage and activating downstream host immune responses. Here we identify and characterize a novel G protein-coupled receptor required to regulate the response to infection with We show that this receptor, which we designate pathogen clearance-defective receptor 1 (PCDR-1), is required for efficient pathogen clearance following infection. PCDR-1 acts upstream of multiple G proteins, including the Gαq ortholog, EGL-30, in rectal epithelial cells to promote pathogen clearance via a novel mechanism.