A new cocktail for liquid scintillation counting of aqueous radioimmunoassay-samples

The aim of this investigation was to find a scintillation mixture for counting up to 1 ml of plasma. We succeeded in obtaining a mixture of xylene/Triton X-100/mono- and di-butylphosphate, and called it Plasmasol. The properties were ideal for radioimmunoassays of steroids. Good dissolving propertie...

Full description

Saved in:
Bibliographic Details
Published inClinica chimica acta Vol. 59; no. 3; pp. 347 - 356
Main Authors Wiegman, T., Woldring, M.G., Pratt, J.J.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 24.03.1975
Subjects
Blood
Carbon Radioisotopes - analysis
Drug Stability
Evaluation Studies as Topic
Indicators and Reagents
Organophosphorus Compounds
Polyethylene Glycols
Radioimmunoassay
Solubility
Steroids - analysis
Testosterone - analysis
Tritium - analysis
Xylenes
Online AccessGet full text

Cover

More Information
Summary:The aim of this investigation was to find a scintillation mixture for counting up to 1 ml of plasma. We succeeded in obtaining a mixture of xylene/Triton X-100/mono- and di-butylphosphate, and called it Plasmasol. The properties were ideal for radioimmunoassays of steroids. Good dissolving properties, high counting efficiency, absence of chemiluminescence and a good stability and reproducibility with respect to the disintegrations per minute were achieved. The cost was only 25% of the commercially available ready-to-use liquid scintillation mixtures, with which a comparison was made.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(75)90010-8