Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections

• Published in: Turkish journal of pediatrics Vol. 59; no. 4; pp. 395 - 403
• Main Authors: Karbuz, Adem, Karahan, Zeynep Ceren, Aldemir-Kocabaş, Bilge, Tekeli, Alper, Özdemir, Halil, Güriz, Haluk, Gökdemir, Refik, İnce, Erdal, Çiftçi, Ergin
• Format: Journal Article
• Language: English
• Published: Turkey Hacettepe University Faculty of Medicine 2017
• Subjects: Adolescent; Anti-Bacterial Agents - pharmacology; Antibiotics; Bacterial Toxins; Child; Child, Preschool; Cloning; Community-Acquired Infections - drug therapy; Community-Acquired Infections - microbiology; Cross Infection - drug therapy; Cross Infection - microbiology; Deoxyribonucleic acid; DNA; Drug Resistance, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field; Enterotoxins - genetics; Exotoxins; Female; Genes; Health care; Humans; Infant; Infant, Newborn; Leukocidins; Male; Pediatrics; Penicillin; Staphylococcal Infections; Staphylococcus aureus - drug effects; Staphylococcus aureus - pathogenicity; Staphylococcus infections; Statistical analysis; Studies; Systematic review; Virulence Factors - genetics; Turkey; pediatric infections; pulsed field gel electrophoresis profile; Staphylococcus aureus; virulence factors
• ISSN: 0041-4301; 2791-6421
• DOI: 10.24953/turkjped.2017.04.005

Karbuz A, Karahan ZC, Aldemir-Kocabaş B, Tekeli A, Özdemir H, Güriz H, Gökdemir R, İnce E, Çiftçi E. Evaluation of antimicrobial susceptibilities and virulence factors of Staphylococcus aureus strains isolated from community-acquired and health-care associated pediatric infections. Turk J Pediatr 2017; 59: 395-403. The aim of this study was to investigate the enterotoxins and Panton-Valentine leukocidin (PVL) gene as virulence factor, identification if antimicrobial sensitivity patterns, agr (accessory gene regulator) types and sequence types and in resistant cases to obtain SCCmec (staphylococcal cassette chromosome mec) gene types which will be helpful to decide empirical therapy and future health politics for S. aureus species. Total of 150 isolates of S. aureus were isolated from the cultures of the child patients in January 2011 and December 2012. In this study, the penicillin resistance was observed as 93.8%. PVL and mecA was detected positive in 8.7% and in 6% of all S. aureus strains, respectively. Two MRSA (methicillin resistant S.aureus) strains were detected as SCCmec type III and SCCmec type V and five MRSA strains were detected as SCCmec type IV. SET-I and SET-G were the most common detected enterotoxins. In both community-associated and healthcare-associated MRSA strains, agr type 1 was detected most commonly. The most common sequence types were ST737 in 13 patients than ST22 in eight patients and ST121 in six patients. This study highlights a necessity to review the cause of small changes in the structural genes in order to determine whether it is a cause or outcome; community-acquired and healthcare associated strains overlap.