Biomolecular alterations in the early life stages of four food fish following acute exposure of Triclosan

• Published in: Environmental toxicology and pharmacology Vol. 91; p. 103820
• Main Authors: Dar, Owias Iqbal, Aslam, Raouf, Sharma, Sunil, Jia, Ai-Qun, Kaur, Arvinder, Faggio, Caterina
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2022; Elsevier Science Ltd
• Subjects: Amino acids; Animals; Biomolecules; Carps - metabolism; Curve fitting; Cyprinidae - metabolism; Early life stages; Embryos; Exposure; Fish; Food fish; Gel electrophoresis; Helices; Infrared spectroscopy; Labeo rohita; Larvae; Polypeptides; Proteomics; Sodium lauryl sulfate; Spectroscopy, Fourier Transform Infrared; Triclosan; Triclosan - toxicity; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Triclosan; Early life stages; Food fish; Proteomics
• ISSN: 1382-6689; 1872-7077
• DOI: 10.1016/j.etap.2022.103820

We investigated the effect of acute concentrations of triclosan (TCS; 96 h exposure and 10d post exposure) on the free amino acid, primary (SDS-PAGE) and secondary (FT-IR) structure of proteins in the embryos/larvae of Cyprinus carpio, Ctenopharyngodon idella, Labeo rohita and Cirrhinus mrigala. A concentration dependent increase in free amino acids, upregulation of polypeptides (100 and 70 kDa in C. carpio, C. idella and L. rohita, 55, 45, 36 kda in C. idella and L. rohita and 22 kDa in all the fish) and a decline in percent area of all the selected peaks of the FT-IR spectra was observed after exposure and recovery period. The decline in percent area was greatest for L. rohita at peak 1080 – 1088 cm−1 (−75.99%) after exposure and at peak 2854 – 2855 cm−1 (−53.59%) after recovery. Curve fitting analysis revealed a decrease in α-helices and increase in β-sheets in all fish after exposure and recovery period. The results suggest that TCS elicits alterations in biomolecules of fish embryos. •Effect of triclosan exposure on protein structure of embryos/ larvae of four food fishes has been investigated.•A concentration dependent rise of amino acid content after exposure and recovery period was observed.•Acute exposure of triclosan induced proteomic and biomolecular alterations in fish embryos/larvae.