Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery

Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength,...

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Published inBiotechnology and bioprocess engineering Vol. 28; no. 1; pp. 83 - 90
Main Authors Kim, Hongbin, Han, Seungmin, Choi, Kyoungcheol, Lee, Jihyeon, Lee, Seung-Hwan, Won, Young-Wook, Lim, Kwang Suk
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Biotechnology and Bioengineering 01.02.2023
Springer Nature B.V
한국생물공학회
Subjects
Biocompatibility
Biodegradability
Biodegradation
Biotechnology
Cellulose
Charge transfer
Chemistry
Chemistry and Materials Science
Conjugation
crystal structure
Deoxyribonucleic acid
divalent metals
DNA
drugs
Gene transfer
genes
Industrial and Production Engineering
Ions
Metal ions
Nanoclusters
Nanocrystals
nanoparticles
phosphates
Research Paper
Self-assembly
Transfection
Ultrafines
Zinc
생물공학
gene delivery
nanocellulose
nanocluster
cellulose nanocrystal
zinc
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Summary:Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength, it is being applied in various fields such as pharmaceuticals and food. In this study, we developed CNC/Zinc/DNA (CZD) nanocomplex using a method to incorporate CNC into DNA without the need for chemical conjugations by utilizing a ligand-to-metal charge transfer transition (LMCT), which occurs between divalent metal ions and the phosphate groups in CNC and DNA. Divalent metal ions should be first introduced to DNA for application of the LMCT transition to the incorporation of DNA into CNC. We optimized the conditions for CZD formation and confirmed gene transfection efficacy in the myocardial and skin cells. DNA was effectively delivered into cells by CZD and highly expressed in the cell. This complex using CNC might be useful as a gene delivery system without chemical conjugation.
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ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-022-0196-4