Instrumentation to rapidly acquire fluorescence wavelength-time matrices of biological tissues

A fiber-optic system was developed to rapidly acquire tissue fluorescence wavelength-time matrices (WTMs) with high signal-to-noise ratio (SNR). The essential system components (473 nm microchip laser operating at 3 kHz repetition frequency, fiber-probe assemblies, emission monochromator, photomulti...

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Bibliographic Details
Published inBiomedical optics express Vol. 1; no. 2; pp. 574 - 586
Main Authors Lloyd, William R, Wilson, Robert H, Chang, Ching-Wei, Gillispie, Gregory D, Mycek, Mary-Ann
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 01.09.2010
Subjects
Spectroscopic Diagnostics
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Summary:A fiber-optic system was developed to rapidly acquire tissue fluorescence wavelength-time matrices (WTMs) with high signal-to-noise ratio (SNR). The essential system components (473 nm microchip laser operating at 3 kHz repetition frequency, fiber-probe assemblies, emission monochromator, photomultiplier tube, and digitizer) were assembled into a compact and clinically-compatible unit. Data were acquired from fluorescence standards and tissue-simulating phantoms to test system performance. Fluorescence decay waveforms with SNR > 100 at the decay curve peak were obtained in less than 30 ms. With optimized data transfer and monochromator stepping functions, it should be feasible to acquire a full WTM at 5 nm emission wavelength intervals over a 200 nm range in under 2 seconds.
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ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.1.000574