A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation

The present work aims to develop a growth medium to render a wild-type strain of Saccharomyces cerevisiae permeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with $3.0\times 10;{-3}$ % SDS is employed. When Brefeldin A is...

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Published inBioMed research international Vol. 2004; no. 3; pp. 150 - 155
Main Authors Pannunzio, Vanesa G., Burgos, Hilda I., Alonso, Manuel, Mattoon, James R., Ramos, Eugenia H., Stella, Carlos A.
Format Journal Article
LanguageEnglish
Published United States Hindawi Publishing Corporation 2004
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Summary:The present work aims to develop a growth medium to render a wild-type strain of Saccharomyces cerevisiae permeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with $3.0\times 10;{-3}$ % SDS is employed. When Brefeldin A is added to this medium, a wild-type strain shows increased growth sensitivity and a diminished transport of the amino acid L-leucine. Since Brefeldin A exerts its effect on the endoplasmic reticulum and the Golgi apparatus, the medium permits the study of the drug effect on the intracellular traffic of L-leucine permeases.
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ISSN:2314-6133
1110-7243
1110-7251
2314-6141
DOI:10.1155/S1110724304308077