Interaction of human osteoblast-like Saos-2 cells with stainless steel coated by silicalite-1 films

• Published in: Materials Science & Engineering C Vol. 76; pp. 775 - 781
• Main Authors: Jirka, Ivan, Vandrovcová, Marta, Plšek, Jan, Bouša, Milan, Brabec, Libor, Dragounová, Helena, Bačáková, Lucie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2017; Elsevier BV
• Subjects: Alkaline phosphatase; Biocompatibility; Cell Adhesion; Cell Line; Cells; Chemical composition; Chemical compounds; Contact angle; Crystals; Electron microscopy; Heat treatment; Human osteoblast-like Saos-2 cells; Humans; Materials science; Microscopy, Electron, Scanning; Osteoblasts; Photoelectron Spectroscopy; Scanning electron microscopy; Silica; Silicalite; Silicalite-1 film; Silicon Dioxide; Spectroscopy; Spreading; Stainless Steel; Stainless steels; Surface Properties; Topology; X ray photoelectron spectroscopy; Human osteoblast-like Saos-2 cells; Biocompatibility; Silicalite-1 film
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2017.03.067

This paper investigates the interaction of human osteoblast-like Saos-2 cells with stainless steel covered by a film of densely inter-grown silicalite-1 crystals with defined outer and inner surfaces. The chemical composition of this film, labeled as SF(RT), was tuned by heat treatment at 300°C and 500°C (labeled as SF(300) and SF(500), respectively) and characterized by X-ray photoelectron spectroscopy (XPS), water drop contact angle (WCA) measurements and scanning electron microscopy (SEM). The number, the spreading area and the activity of alkaline phosphatase of human osteoblast-like Saos-2 cells in cultures on the silicalite-1 film were affected by the chemical composition of its outer surface and by its micro-porous structure. The number and the spreading area of the adhered osteoblast-like cells on day 1 was highest on the surface of SF(RT) relative to their adhesion and spreading on a glass cover slip due to the SF(RT) topology. However, SF(300) markedly supported cell growth during days 3 and 7 after seeding. [Display omitted] •Bioactivity of a,b silicalite-1 films (SF) with characterized surface is investigated•The number, spreading area and activity of alkaline phosphatase of human osteoblast-like Saos-2 cells in cultures on SF is dependent on chemical composition and accessibility of microporous structure of the film.•The number of adhered osteoblast-like Saos-2 cells can be tuned by chemical composition of outer SF film.