Indole-3-acetic Acid Production from Alginate by Vibrio sp. dhg: Physiology and Characteristics

• Published in: Biotechnology and bioprocess engineering Vol. 28; no. 4; pp. 695 - 703
• Main Authors: Shin, Hyo Jeong, Woo, Sunghwa, Jung, Gyoo Yeol, Park, Jong Moon
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.08.2023; Springer Nature B.V; 한국생물공학회
• Subjects: Acetic acid; Acid production; Algae; Alginates; Alginic acid; Bacteria; Biosynthesis; Biotechnology; Carbon; Carbon sources; Chemistry; Chemistry and Materials Science; glucose; Growth regulators; indole acetic acid; Indoleacetic acid; Industrial and Production Engineering; Mannitol; Microorganisms; Nicotinamide adenine dinucleotide; Plant growth; Research Paper; Tryptophan; Vibrio; 생물공학; alginate; indole-3-acetic acid (IAA); L-tryptophan
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-023-0056-x

The production of indole-3-acetic acid (IAA) by bacteria has attracted considerable attention in plant studies due to its significant role as a plant growth regulator. In this study, it was confirmed that Vibrio sp. dhg, a novel microbial platform capable of assimilating alginate, can naturally synthesize IAA. The effects of L-tryptophan and the carbon sources obtained from brown algae (glucose, mannitol, and alginate) were also examined to characterize the IAA biosynthesis in Vibrio sp. dhg. The highest IAA production (9.32 ± 0.25 mg/L) was observed in the alginate medium containing 0.8 g/L of L-tryptophan. Interestingly, alginate was found to be a favorable option for IAA production due to the rapid uptake of L-tryptophan during the exponential phase. By adding external NADH, this study demonstrated that the low net reducing equivalents in the alginate medium were linked to this phenomenon. This study is the first to provide alginate as the sole carbon source for IAA production and to propose that the oxidoreduction potentials of the carbon source can affect bacterial IAA biosynthesis.