Progress in Top-Down LC-MS Analysis of Antibodies: Review

Antibodies are large heterogenous proteins due to post-translational modifications, including glycosylation. Structural analysis of antibodies by mass spectrometry has been of great interest recently, especially in the pharmaceutical industry. Over the past decade, bottom-up mass spectrometry has be...

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Bibliographic Details
Published inBiotechnology and bioprocess engineering Vol. 28; no. 1; pp. 226 - 233
Main Authors You, Jiwon, Park, Hae-Min
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Biotechnology and Bioengineering 01.02.2023
Springer Nature B.V
한국생물공학회
Subjects
Antibodies
Biotechnology
Chemistry
Chemistry and Materials Science
Glycosylation
Industrial and Production Engineering
Ionization
Ions
Mass spectrometry
Mass spectroscopy
Peptides
Pharmaceutical industry
Post-translation
Proteins
Proteolysis
proteome
Proteomes
Review Paper
Scientific imaging
Structural analysis
생물공학
antibody
intact proteins
post-translational modifications
top-down mass spectrometry
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Summary:Antibodies are large heterogenous proteins due to post-translational modifications, including glycosylation. Structural analysis of antibodies by mass spectrometry has been of great interest recently, especially in the pharmaceutical industry. Over the past decade, bottom-up mass spectrometry has been widely used to identify single protein targets and whole proteomes from a biological system such as a cell, tissue, or organism. However, due to the protein inference problem in bottom-up mass spectrometry, this approach has intrinsic limitations for characterizing the post-translationally modified forms of proteins such as antibodies. In contrast to bottom-up mass spectrometry measuring peptides produced from proteins by proteolytic digestion, top-down mass spectrometry is a powerful technology that allows measuring proteins without proteolysis, thus characterizing intact forms of proteins, which provides information on primary sequences, including modifications characterization. In this review, we outline some progress in the separation, ionization, and fragmentation of intact proteins for top-down mass spectrometry and the growing power of intact protein-resolved measurements in biotechnology.
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ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-023-0011-x