Relationship between lipid peroxidation and rigidity in L- α-phosphatidylcholine-DPPC vesicles

• Published in: Journal of colloid and interface science Vol. 323; no. 1; pp. 70 - 74
• Main Authors: Soto-Arriaza, M.A., Sotomayor, C.P., Lissi, E.A.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.07.2008; Elsevier
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Anisotropy; Chemistry; Colloidal state and disperse state; Egg-PC-DPPC vesicles; Exact sciences and technology; Fluorescence Polarization; General and physical chemistry; Lipid Bilayers; Lipid Peroxidation; Lipids - chemistry; Liposomes - chemistry; Membrane Fluidity; Membrane rigidity; Membranes; Microscopy, Fluorescence - methods; Oxygen - metabolism; Oxygen Consumption; Permeability; Peroxides - metabolism; Phosphatidylcholines - chemistry; Surface physical chemistry; Water - chemistry; Water transport; Egg-PC; LP; AAPH; Lipid peroxidation; GP; Membrane rigidity; MDA; LUV; TMA-DPH; TCA; Egg-PC-DPPC vesicles; Water transport; Laurdan; DPH; DPPC; Water; Vesicle; Egg; Phosphatidylcholine; Lipids; Membrane; Transport; Peroxidation
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2008.04.034

DPPC incorporation into egg-PC unilamellar vesicles reduces their oxidation rate beyond that expected from the unsaturated lipid dilution. Addition of the unsaturated lipids produces changes in the physical properties of the inner parts of the lipid bilayer, as sensed by fluorescence anisotropy of DPH, and in the hydrophilic/hydrophobic region, as sensed by the generalized polarization of laurdan. DPPC (30 mol%) incorporation into egg-PC vesicles produces a decrease in alkyl chain mobility in the inner part of the bilayer, evaluated by the increase of DPH fluorescence anisotropy, and a rise of the generalized polarization value of laurdan in the bilayer interface. It also leads to a decrease in the rate of water efflux promoted by a hypertonic shock. Oxidation of PC LUVs, promoted by AAPH, as sensed by oxygen uptake and MDA formation, leads to qualitatively similar results than DPPC addition: rigidification at the inner part and the surface of the liposomes, and a lower rate of water permeation. It is suggested that these changes could contribute to the observed decrease in oxidation rate with conversion. The DPPC incorporation into egg-PC vesicles produces changes in the physical properties of the inner parts and in the hydrophilic/hydrophobic region of the lipid bilayer. The addition produces a decrease in alkyl chain mobility in the inner part of the bilayer and a decrease in the rate of water efflux promoted by a hypertonic shock. The same effect is produced when the oxidation of egg-PC LUVs is carried out, rigidification of the inner part and the surface of the liposomes, and a lower rate of water permeation.