Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota

• Published in: The journal of microbiology Vol. 60; no. 9; pp. 905 - 915
• Main Authors: Zhang, Dao-Feng, Yao, Yu-Fang, Xue, Hua-Peng, Fu, Zi-Yue, Zhang, Xiao-Mei, Shao, Zongze
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.09.2022; Springer Nature B.V; 한국미생물학회
• Subjects: Autoimmunity; Bacteria; Biomedical and Life Sciences; Catalase; Cell culture; Chromosomes; CRISPR; DNA sequencing; Fatty acids; Flagella; Gene clusters; Gene editing; Gene sequencing; Genomes; Life Sciences; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleotide sequence; Quinones; rRNA 16S; Sodium chloride; Taxonomy; 생물학; gen. nov; intertidal zone; sp. nov; CRISPR-Cas systems
• ISSN: 1976-3794; 1225-8873; 1976-3794
• DOI: 10.1007/s12275-022-2102-3

A novel bacterium, designated YYF0007 T , was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007 T had highest 16S rRNA gene sequence similarity (92.2%) to Jiulongibacter sediminis JN-14-9 T . The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007 T should be recognized as a novel genus of the family Spirosomaceae. The cells were Gramstain-negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0–3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C 16:1 ω 5 c , iso-C 15:0 and summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family Spirosomaceae and the phylum Bacteroidota indicated that it was remarkable for strain YYF0007 T featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF-0007 T in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name Marinilongibacter aquaticus gen. nov., sp. nov. is proposed, and the type strain is YYF0007 T (= MCCC 1K06017 T = GDMCC 1.2428 T = JCM 34683 T ).