Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota
A novel bacterium, designated YYF0007 T , was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated t...
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Published in | The journal of microbiology Vol. 60; no. 9; pp. 905 - 915 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Seoul
The Microbiological Society of Korea
01.09.2022
Springer Nature B.V 한국미생물학회 |
Subjects | |
Online Access | Get full text |
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Summary: | A novel bacterium, designated YYF0007
T
, was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007
T
had highest 16S rRNA gene sequence similarity (92.2%) to
Jiulongibacter sediminis
JN-14-9
T
. The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007
T
should be recognized as a novel genus of the family
Spirosomaceae.
The cells were Gramstain-negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0–3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C
16:1
ω
5
c
, iso-C
15:0
and summed feature 3 (C
16:1
ω
7
c
and/or C
16:1
ω
6
c
). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family
Spirosomaceae
and the phylum
Bacteroidota
indicated that it was remarkable for strain YYF0007
T
featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF-0007
T
in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name
Marinilongibacter aquaticus
gen. nov., sp. nov. is proposed, and the type strain is YYF0007
T
(= MCCC 1K06017
T
= GDMCC 1.2428
T
= JCM 34683
T
). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1976-3794 1225-8873 1976-3794 |
DOI: | 10.1007/s12275-022-2102-3 |