Controlled antifungal behavior on Ti6Al4V nanostructured by chemical nanopatterning

• Published in: Materials Science & Engineering C Vol. 96; pp. 677 - 683
• Main Authors: Valdez-Salas, Benjamín, Beltrán-Partida, Ernesto, Nedev, Nicola, Ibarra-Wiley, Roberto, Salinas, Ricardo, Curiel-Álvarez, Mario, Valenzuela-Ontiveros, Yaneth, Pérez, Guillermo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2019; Elsevier BV
• Subjects: Adhesion tests; Anatase; Antimicrobial; Candida albicans; Cell surface; Colonization; Dental implants; Dental materials; Dental prosthetics; Dental restorative materials; Exopolysaccharides; Fabrication; Fungi; Fungicides; Materials science; Metallic biomaterials; Nanopatterning; Nanostructure; Nanostructures; Organic chemistry; Porosity; Prostheses; Prosthetics; Scanning electron microscopy; Secretion; Substrates; Surgical implants; Titanium; Titanium base alloys; Titanium dioxide; Transplants & implants; Viability; Yeast; Antimicrobial; Candida albicans; Titanium; Metallic biomaterials; Nanostructures; Dental implants
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2018.11.086

Infections associated with bone implant prostheses are mainly related to bacterial contaminations. Recent investigations have suggested an important role of opportunistic fungal cells associated with non-responding antibacterial treatments. Thus, in order to evaluate the early Candida albicans (C. albicans) behavior; we built on Ti6Al4V surfaces nanopores (NPs) with controlled diameters applying oxidative nanopatterning for 30 (NP30) and 60 min (NP60). As a result of nanopatterning NPs with diameters of 12 and 24 nm were synthesized. Physicochemical differences were observed between both types of NPs, the most highlighting of which are anatase phase formation and improved hydrophilicity of NP60. C. albicans adhesion and colonization was assessed using scanning electron microscopy and by yeast counting for viability evaluation. The fungal behavior on the substrates was significantly different, showing an initial exopolysaccharide secretion stimulated by the nanopatterned surfaces. Larger NPs led to an important reduction in viability with decreased cell-surface contact bonds. The obtained results demonstrate that special control in the fabrication of nanostructured TiO2 materials can improve the early fungal resistance, especially for dental implants. [Display omitted] •The synthesis of nanostructure pores reduced the adhesion of C. albicans.•Anatase and hydrophilic TiO2 nanopores promotes antifungal capability.•The results showed different fungal behaviors on the Ti6Al4V experimental surfaces.•The control in the nanopores size promise to regulate the fungal activity.