Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 4; pp. 713 - 716
• Main Authors: Cui, Ying-Shun, Im, Wan-Taek, Yin, Cheng-Ri, Yang, Deok-Chun, Lee, Sung-Taik
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.04.2007; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Korea; Microbiology; Molecular Sequence Data; Panax - microbiology; Phylogeny; Propionibacteriaceae; Propionibacteriaceae - classification; Propionibacteriaceae - genetics; Propionibacteriaceae - isolation & purification; Propionibacteriaceae - physiology; RNA, Ribosomal, 16S - genetics; Soil Microbiology; Systematics; Asia; South Korea; Korea; Tropical crop; Soils; Bacteria; Taxonomy; Cultivated soil
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64395-0

1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea 2 Key Laboratory of Organism Functional Factors of the Changbai Mountain, Ministry of Education, Yanbian University, Yanji 133002, People's Republic of China 3 Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, Republic of Korea Correspondence Wan-Taek Im wandra{at}kaist.ac.kr A Gram-positive, aerobic, coccus-shaped, non-endospore-forming bacterium (Gsoil 633 T ) was isolated from soil from a ginseng field in Pocheon province in South Korea. The novel isolate was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 633 T was shown to belong to the family Propionibacteriaceae . The closest phylogenetic relative was Microlunatus phosphovorus DSM 19555 T , with 96.1 % sequence similarity; the sequence similarity to other members of the family was less than 95.4 %. The isolate was characterized chemotaxonomically as having LL -2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H 4 ) as the predominant menaquinone and anteiso-C 15 : 0 , iso-C 15 : 0 and iso-C 16 : 0 as the major fatty acids. The G+C content of the genomic DNA was 69.8 mol%. The morphological and chemotaxonomic properties of the isolate were consistent with those of M. phosphovorus , but the results of physiological and biochemical tests allowed the phenotypic differentiation of strain Gsoil 633 T from this species. Therefore, strain Gsoil 633 T represents a novel species, for which the name Microlunatus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 633 T (=KCTC 13940 T =DSM 17942 T ). Abbreviations: LL -DAP, LL -2,6-diaminopimelic acid The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Gsoil 633 T is AB245389 .