Bright fluorescent nanoparticles for developing potential optical imaging contrast agents

Fluorescent cross-linked nanoparticles with variable fluorophore loading amounts, locations, and particle sizes were synthesized from sequential one-pot functionalization/cross-linking of block copolymer micelles with amine-terminated dye and cross-linker molecules, via reductive amination and amida...

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Bibliographic Details
Published inNanoscale Vol. 2; no. 4; pp. 548 - 558
Main Authors Sun, Guorong, Berezin, Mikhail Y, Fan, Jinda, Lee, Hyeran, Ma, Jun, Zhang, Ke, Wooley, Karen L, Achilefu, Samuel
Format Journal Article
LanguageEnglish
Published England 01.01.2010
Subjects
Brightness
Contrast agents
Contrast Media - chemistry
Crosslinking
Dyes
Fluorescence
Fluorescent Dyes - chemistry
Imaging
Micelles
Microscopy, Electron, Transmission
Nanoparticles
Nanoparticles - chemistry
Nanostructure
Particle Size
Quantum Theory
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Summary:Fluorescent cross-linked nanoparticles with variable fluorophore loading amounts, locations, and particle sizes were synthesized from sequential one-pot functionalization/cross-linking of block copolymer micelles with amine-terminated dye and cross-linker molecules, via reductive amination and amidation. The fluorescence quantum yield and brightness of these nanoparticles were evaluated by steady-state and dynamic fluorescence methods. The results demonstrate that the quantum yield and brightness of the fluorescent nanoparticles correlated directly with the number of dyes/nanoparticle and the nanoparticle size. A strategy to increase the fluorescence brightness of nanoparticles with fluorescein and near-infrared dyes is proposed.
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ISSN:2040-3364
2040-3372
DOI:10.1039/b9nr00304e