Determination of tilmicosin in swine feeds by liquid chromatography
This method determines tilmicosin in feeds over a concentration range of 100 to 600 mg/kg. Tilmicosin is extracted from swine feeds by adding 200 ml of a swine feed extractant (20 + 80, acetonitrile-Millipore water, pH 2.5, with 25 mM dibutylammonium phosphate) to 20 g feed and placing on a shaker t...
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Published in | Journal of AOAC International Vol. 80; no. 6; p. 1161 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
01.11.1997
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Subjects | |
Online Access | Get more information |
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Summary: | This method determines tilmicosin in feeds over a concentration range of 100 to 600 mg/kg. Tilmicosin is extracted from swine feeds by adding 200 ml of a swine feed extractant (20 + 80, acetonitrile-Millipore water, pH 2.5, with 25 mM dibutylammonium phosphate) to 20 g feed and placing on a shaker table for 1 h. This extractant is filtered and analyzed by liquid chromatography (LC). A gradient LC method is used to separate tilmicosin from the feed matrix in 30 min of run time. The recovery of tilmicosin from fortified feeds ranged from 96.7 to 112%, with the coefficients of variation (CVs) ranging from 1.4 to 3.9%. The determination of tilmicosin in medicated feeds resulted in an average recovery of 92.7% of labeled claim for pelleted feeds at 200 mg/kg and 99.1% of labeled claim for mash feeds at 400 mg/kg. Determination of tilmicosin in medicated feeds resulted in CVs ranging from 2.6 to 3.8%. The method has shown no interference with 18 other drugs |
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Bibliography: | 1997070802 Q54 |
ISSN: | 1060-3271 1944-7922 |
DOI: | 10.1093/jaoac/80.6.1161 |