Unravelling the human triple negative breast cancer suppressive activity of biocompatible zinc oxide nanostructures influenced by Vateria indica (L.) fruit phytochemicals

• Published in: Materials Science & Engineering C Vol. 122; p. 111887
• Main Authors: D'Souza, Josline Neetha, Prabhu, Ashwini, Nagaraja, G.K., Navada K., Meghana, Kouser, Sabia, Manasa, D.J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2021; Elsevier BV
• Subjects: Animals; Anticancer properties; Antitumor activity; Apoptosis; Biocompatibility; Biosynthesis; Breast cancer; Cell Line, Tumor; Chemical composition; Cytotoxicity; Dipterocarpaceae; Erythrocytes; Fluorescence; Fruit; Fruits; Humans; Infrared spectroscopy; Intracellular levels; Materials science; Mice; Morphology; Nanostructure; Nanostructures; NIH 3T3 Cells; Oxidative stress; Phytochemicals; Phytochemicals - pharmacology; Reactive oxygen species; Spectroscopy; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Stoichiometry; TNBC cytotoxicity; Toxicity; Triple Negative Breast Neoplasms - drug therapy; V. indica; Zinc Oxide; ZnOVI nanostructures; Oxidative stress; ZnOVI nanostructures; Biocompatibility; V. indica; TNBC cytotoxicity
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2021.111887

The present study delineates the biosynthesis of ZnOVI nanostructures by using aqueous fruit extract of V. indica. The study has disclosed the role of V. indica fruit extract as both reducing and capping agents, ushering the formation of ZnOVI nanostructures with distinct morphologies. The formation of ZnOVI nanostructures was corroborated by FT-IR and UV–visible spectroscopy which was further substantiated by the elemental composition study through EDS spectroscopy. The nanostructures were also investigated by Rietveld refinement of PXRD data, FE-SEM, and BET analysis. The morphology, size, and surface area were found to be precursor stoichiometry dependent. The in-vitro cytotoxicity study of ZnOVI nanostructures carried out on MDA-MB468 human triple-negative breast cancer (TNBC) cells has revealed their potential cytotoxicity (91.18 ± 1.98). MTT assay performed on the NIH3T3 mouse fibroblast cells has unfolded the non-toxic nature of ZnOVI nanostructures. Additionally, the results of the AO-EB dual staining assay indicated early apoptosis in TNBC cells by displaying greenish yellow-fluorescence in the nuclei. Reactive oxygen species (ROS) measurement study has confirmed the elevated intracellular levels of ROS, supporting the oxidative-stress induced cytotoxicity in ZnOVI nanostructures treated TNBC cells. Furthermore, the haemocompatibility of ZnOVI nanostructures was evaluated using human erythrocytes. Thus, the obtained results have shown greater potential in the anticancer activity of bio-fabricated ZnOVI nanostructures. [Display omitted] •Bio-compatible ZnOVI nanostructures were biosynthesized via Vateria indica (VI) fruit extract.•The structural and morphological characterizations have revealed the formation of ZnOVI nanostructure with distinct morphologies and size not more than 32 nm.•ZnOVI nanostructures exhibited up to 91.18±1.98 % human triple negative breast cancer suppressive activity.•ZnOVI nanostructures used as nanocatalyst showed impressive MB dye degradation property with an excellent stability and reusability.