Corn silage-based diet supplemented with increasing amounts of linseed oil: Effects on methane production, rumen fermentation, nutrient digestibility, nitrogen utilization, and milk production of dairy cows

• Published in: Journal of dairy science Vol. 104; no. 5; pp. 5375 - 5390
• Main Authors: Hassanat, F., Benchaar, C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2021
• Subjects: Animals; Cattle; corn silage; Diet - veterinary; Dietary Supplements; Digestion; Female; Fermentation; Lactation; linseed oil; Linseed Oil - metabolism; methane; Methane - metabolism; Nitrogen - metabolism; Nutrients; Rumen - metabolism; rumen fermentation; Silage - analysis; Zea mays; methane; rumen fermentation; corn silage; linseed oil
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2020-18853

In this study, we assessed the effects of increasing amounts of linseed oil (LSO) in corn silage-based diets on enteric CH4 production, rumen fermentation characteristics, protozoal population, nutrient digestibility, N utilization, and milk production. For this purpose, 12 multiparous lactating Holstein cows (84 ± 28 d in milk; mean ± SD) fitted with ruminal cannula were used in a replicated 4 × 4 Latin square design (35-d period). The cows were fed ad libitum a total mixed ration without supplementation (control) or supplemented [on a dry matter (DM) basis] with LSO at 2% (LSO2), 3% (LSO3) or 4% (LSO4). The forage:concentrate ratio was 61:39 (on DM basis) and was similar among the experimental diets. The forage portion consisted of corn silage (58% diet DM) and timothy hay (3% diet DM). The proportions of soybean meal, corn grain and soybean hulls decreased as the amount of LSO in the diet increased. Daily methane production (g/d) decreased quadratically as the amount of LSO increased in the diet. Increasing LSO dietary supplementation caused a linear decrease in CH4 emissions expressed on either DM intake (DMI) basis (−9, −20, and −28%, for LSO2, LSO3, and LSO4, respectively) or gross energy intake basis (−12, −22, and −31%, for LSO2, LSO3, and LSO4, respectively). At 2 and 3% LSO, the decrease in enteric CH4 emissions occurred without negatively affecting DMI or apparent total-tract digestibility of fiber and without changing protozoa numbers. However, these 2 diets caused a shift in volatile fatty acids pattern toward less acetate and more propionate. The effect of the LSO4 diet on enteric CH4 emissions was associated with a decrease in DMI, fiber apparent-total-tract digestibility, protozoa numbers (total and genera), and an increase in propionate proportion at the expense of acetate and butyrate proportions. Methane emission intensity [g of CH4/kg of energy-corrected milk (ECM)] decreased linearly (up to 28% decrease) with increasing LSO level in the diet. Milk fat yield decreased linearly (up to 19% decrease) with increasing inclusion of LSO in the diet. Milk protein yield increased at 2% or 3% LSO and decreased to the same level as that of the nonsupplemented diet at 4% LSO (quadratic effect). Yield of ECM was unchanged by LSO2 and LSO3 treatments but decreased (−2.8 kg/d) upon supplementation with 4% LSO (quadratic effect). Efficiency of milk production (kg ECM/kg DMI) was unaffected by the 3 levels of LSO. Ruminal NH3 concentration was quadratically affected by LSO supplementation; decreasing only at the highest level of LSO supplementation. The amount (g/d) of N excreted in feces and urine decreased linearly and quadratically, respectively, as the amount of LSO increased in the diet, mainly because of the reduction in N intake. Efficiency of dietary N used for milk N secretion increased linearly with increasing LSO supplementation in the diet. We conclude that supplementing corn silage-based diets with 2 or 3% of LSO can reduce enteric CH4 emissions up by to 20% without impairing animal productivity (i.e., ECM yield and feed efficiency).