Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter

Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin co...

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Published inBiomedical optics express Vol. 9; no. 2; pp. 518 - 528
Main Authors Shi, Yaru, Liu, Wei, Zheng, Haifeng, Li, Zhiqiang, Shi, Xiaorui, Cai, Shixuan, Jiao, Zhiqiang, Mao, Wenjie, Xie, Jinrong, Tian, Jie, Wang, Fu
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 01.02.2018
Subjects
(170.6280) Spectroscopy, fluorescence and luminescence
(170.3880) Medical and biological imaging
(170.0170) Medical optics and biotechnology
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Summary:Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing.
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ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.9.000518