Ultrasound- and Negative Pressure-assisted Fractional Precipitation of Paclitaxel from Taxus chinensis

• Published in: Biotechnology and bioprocess engineering Vol. 28; no. 2; pp. 336 - 344
• Main Authors: Shin, Eun-Ju, Kim, Jin-Hyun
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.04.2023; Springer Nature B.V; 한국생물공학회
• Subjects: activation energy; Biotechnology; Chemistry; Chemistry and Materials Science; Diffusion coefficient; Industrial and Production Engineering; Paclitaxel; Precipitates; Pressure; Rate constants; Research Paper; Taxus chinensis; Taxus wallichiana var. chinensis; Ultrasonic imaging; ultrasonics; Ultrasound; 생물공학; paclitaxel; negative pressure; ultrasound; fractional precipitation; synergistic effect
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-022-0364-6

The effect of ultrasound and negative pressure on the efficiency of fractional precipitation of paclitaxel derived from Taxus chinensis was investigated. Introducing both ultrasound and negative pressure during fractional precipitation resulted in a precipitation time that was 2 times faster than the conventional ultrasound-fractional precipitation and negative pressure-fractional precipitation. In particular, using an ultrasonic power of 250 W and a negative pressure of -200 mmHg simultaneously could recover up to 98.2% of the paclitaxel within 1 min. The ultrasound/negative pressure-fractional precipitation resulted in precipitates with mean particle sizes that were 9.3, 2.3, and 2.7 times smaller than those of the conventional fractional precipitation, the negative pressure-fractional precipitation, and the ultrasound-fractional precipitation, respectively. In addition, the diffusion coefficients increased by 11.4, 2.2, and 2.6 times, and the rate constants increased by 2.9–7.0, 1.3–3.0, and 2.3–5.5 times. As the ultrasonic power and negative pressure increased, the changes of activation energy decreased, promoting faster precipitation.