Fast volumetric phase-gradient imaging in thick samples

Oblique back-illumination microscopy (OBM) provides high resolution, sub-surface phase-gradient images from arbitrarily thick samples. We present an image formation theory for OBM and demonstrate that OBM lends itself to volumetric imaging because of its capacity for optical sectioning. In particula...

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Bibliographic Details
Published inOptics express Vol. 22; no. 1; pp. 1152 - 1162
Main Authors David Giese, J, Ford, Tim N, Mertz, Jerome
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 13.01.2014
Subjects
Animals
Cerebral Cortex - cytology
Equipment Design
Equipment Failure Analysis
Imaging, Three-Dimensional - instrumentation
Lenses
Lighting - instrumentation
Mice
Microscopy - instrumentation
Nephelometry and Turbidimetry - instrumentation
Nephelometry and Turbidimetry - methods
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Summary:Oblique back-illumination microscopy (OBM) provides high resolution, sub-surface phase-gradient images from arbitrarily thick samples. We present an image formation theory for OBM and demonstrate that OBM lends itself to volumetric imaging because of its capacity for optical sectioning. In particular, OBM can provide extended depth of field (EDOF) images from single exposures, by rapidly scanning the focal plane with an electrically tunable lens. These EDOF images can be further enhanced by deconvolution. We corroborate our theory with experimental volumetric images obtained from transparent bead samples and mouse cortical brain slices.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.22.001152