Impact of the Synthetic Scaffold Strategy on the Metabolic Pathway Engineering

• Published in: Biotechnology and bioprocess engineering Vol. 28; no. 3; pp. 379 - 385
• Main Authors: Tran, Kim-Ngan T., Kumaravel, Ashokkumar, Hong, Soon Ho
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.06.2023; Springer Nature B.V; 한국생물공학회
• Subjects: Amino acids; biochemical pathways; Biological activity; Biorefineries; biorefining; Biotechnology; Chemistry; Chemistry and Materials Science; E coli; Engineering; Enzymes; Industrial and Production Engineering; Kinases; Ligands; Localization; Metabolic engineering; Metabolic pathways; Metabolism; Metabolites; Peptides; Physiology; Protein engineering; Protein interaction; Proteins; Refineries; Regulatory mechanisms (biology); Review Paper; Scaffolds; Signal transduction; Synthetic biology; Yeast; 생물공학; scaffold protein; synthetic biology; metabolic engineering
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-022-0350-z

For the development of the efficient bio-refinery process or biochemical producer, metabolic engineering has become an attractive choice recently. However, engineered metabolic pathways often suffer from flux imbalances due to a lack of corresponding regulatory mechanisms associated with natural metabolism. The interaction among different enzymes within a metabolic pathway plays an important role in regulating the efficiency of metabolic processes. Consequently, the creation of protein scaffolds has helped with the spatial co-localization of proteins in metabolic engineering. Research on protein scaffolds indicated scaffold systems may enhance metabolic productivity further. In this review, the specificity, selectivity, and regulatory mechanisms of protein-protein interactions are discussed in the context of the important effects that they exert on various biological processes.