The deduced primary structure of a ribosomal protein S4 from Trypanosoma cruzi

• Published in: Biochimica et biophysica acta Vol. 1395; no. 3; pp. 321 - 325
• Main Authors: Hernández, Roberto, Palacios, Sergio, Herrera, Juliana, Martı́nez-Calvillo, Santiago, López, Imelda
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 11.02.1998
• Subjects: American trypanosomiasis; Amino Acid Sequence; amino acid sequences; Animals; Chromosome Mapping; cloning; Cloning, Molecular; comparisons; complementary DNA; Conserved Sequence; DNA, Complementary; genbank/af005421; genbank/af005904; Gene Library; Humans; Molecular Sequence Data; nucleotide sequences; proteins; Protozoan Proteins - biosynthesis; Protozoan Proteins - chemistry; Protozoan Proteins - genetics; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Ribosomal protein S4; Ribosomal Proteins - biosynthesis; Ribosomal Proteins - chemistry; Ribosomal Proteins - genetics; ribosomes; Sequence Alignment; Sequence Homology, Amino Acid; Trypanosoma cruzi; Trypanosoma cruzi - genetics; Trypanosoma cruzi - metabolism; American trypanosomiasis; Ribosomal protein S4; Trypanosoma cruzi
• ISSN: 0167-4781; 0006-3002; 1879-2634; 1878-2434
• DOI: 10.1016/S0167-4781(97)00224-8

Two cDNA clones encoding a protein homologous to ribosomal protein S4 from the protozoan parasite Trypanosoma cruzi were isolated and characterized. Both clones potentially encode for an identical basic protein of 273 amino acids. Sequence comparisons with other species indicate that this protein is highly conserved. Hybridization studies are consistent with the occurrence of two genes in T. cruzi encoding this ribosomal protein. An RNA of approximately 1 kb was present in both exponentially growing and stationary phase derived epimastigotes, and no significant differences were detected in its steady state concentration.