Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

• Published in: Biomedical optics express Vol. 7; no. 1; pp. 45 - 56
• Main Authors: Aekbote, Badri L, Fekete, Tamás, Jacak, Jaroslaw, Vizsnyiczai, Gaszton, Ormos, Pál, Kelemen, Lóránd
• Format: Journal Article
• Language: English
• Published: United States Optical Society of America 01.01.2016
• Subjects: (140.7090) Ultrafast lasers; (230.6120) Spatial light modulators; (160.1435) Biomaterials; (180.2520) Fluorescence microscopy; (160.5470) Polymers; (350.3390) Laser materials processing; (350.4855) Optical tweezers or optical manipulation; (230.4000) Microstructure fabrication; (310.0310) Thin films
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/BOE.7.000045

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.