Neurochemical properties of neurospheres infusion in experimental-induced seizures

• Published in: Tissue & cell Vol. 54; pp. 47 - 54
• Main Authors: de Gois da Silva, Mirna Luciano, da Silva Oliveira, George Laylson, de Oliveira Bezerra, Dayseanny, da Rocha Neto, Hermínio José, Feitosa, Matheus Levi Tajra, Argôlo Neto, Napoleão Martins, Rizzo, Marcia dos Santos, de Carvalho, Maria Acelina Martins
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.10.2018; Elsevier Science Ltd
• Subjects: Animals; Antioxidants; Biomarkers; Brain; Brain - physiopathology; Catalase; Cell therapy; Convulsants - toxicity; Differentiation; Endocrine therapy; Epilepsy; Female; Glutathione; Histopathology; Male; Neural stem cells; Neural Stem Cells - transplantation; Neurochemistry; Neurodegeneration; Neurodegenerative diseases; Neurological diseases; Neurospheres; Oxidative stress; Oxidative Stress - physiology; Pentylenetetrazole; Picrotoxin; Pilocarpine; Pilocarpine - toxicity; Rats; Rats, Wistar; Regeneration; Seizures; Seizures - chemically induced; Seizures - physiopathology; Stem Cell Transplantation - methods; Stem cells; Superoxide dismutase; Therapy; Thiobarbituric acid; Rats; Oxidative stress; Neural stem cells; Cell therapy; Epilepsy
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2018.08.002

•NSCs generated neurospheres with more spherical and dense clusters on the 15th day of cell culture.•Neurospheres are tracked by fluorescence in brain tissue after 30 days of infusion.•Lipid peroxidation and nitrite levels increase in animals with status epilepticus.•GSH, SOD and CAT enzymes rise significantly after neurospheres treatment in PIL group.•Neurospheres remain intact and capable to differentiate into necrotic areas in PIL group. Cell replacement through neural stem cells has been a promising alternative therapy for neurodegenerative diseases. It was evaluated the possible protect and/or prevent role of neurospheres in experimental models of epilepsy by the use of biomarkers of oxidative stress and histopathological analysis. After 1 h of the epileptic inductions by pilocarpine, pentylenotetrazole and picrotoxin, rats were infused with a suspension of 2 × 106 cells/0.25 mL, marked with Qtracker® 655, via caudal vein. In the control group epilepsy was not induced, but received the cell infusion under the same conditions of other groups. After 30 days, the rats were euthanized, and the removal of the brain was proceeded to later perform the assays oxidative stress and histopathology analysis. Thiobarbituric acid and nitrite levels were elevated in epileptic groups treated with neurospheres, and the levels of reduced glutathione, superoxide dismutase and catalase were reduced when compared to non-treated groups. The performance of oxidative enzymes from pilocarpine group treated with neurospheres showed slight increase. Histopathological evaluation observed distribution of neurospheres throughout the brain tissue, with viable cells and in process of differentiation in the pilocarpine group, but with differentiation and regeneration compromised in epilepsy by picrotoxin and pentylenetetrazole due to a microenvironment of oxidative stress. Neural stem cell therapy has a promising potential for protection in the pilocarpine epilepsy model, suggesting that the antioxidant system of neurospheres could reduce oxidative damage generated by seizure.