Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop

• Published in: Veterinary immunology and immunopathology Vol. 60; no. 3; pp. 251 - 260
• Main Authors: Pescovitz, M.D, Book, B.K, Aasted, B, Dominguez, J, Ezquerra, A, Trebichavsky, I, Novikov, B, Valpotic, I, Sver, L, Nielsen, J, Arn, S, Sachs, D.H, Lunney, J.K, Boyd, P.C, Walker, J, Lee, R, Davis, William, Barbosa, I.R, Zuckermann, F, Saalmüller, A
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Netherlands Elsevier B.V 30.01.1998
• Subjects: Animals; Antibodies, Monoclonal - metabolism; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGEN ANTIBODY REACTIONS; ANTIGENE; ANTIGENOS; ANTIGENS; Antigens, CD - immunology; Antigens, Differentiation, T-Lymphocyte - immunology; CD2 Antigens - immunology; CD3 Complex - immunology; CD4 Antigens - immunology; CD5 Antigens - immunology; CD8 Antigens - immunology; CELL COUNTING; CERDO; CONTEO DE CELULAS; LINFOCITOS; LYMPHOCYTE; Lymphocyte Activation; LYMPHOCYTES; MONOCLONAL ANTIBODIES; NUMERATION CELLULAIRE; PORCIN; Porcine; REACCION ANTIGENO ANTICUERPO; REACTION ANTIGENE ANTICORPS; Receptors, Interleukin-2 - immunology; SWINE; Swine - immunology; Swine CD workshop; T-cells; T-Lymphocytes - immunology; T-cells; Monoclonal antibodies; Porcine; Swine CD workshop
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/S0165-2427(97)00101-3

After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assignments included: two CD2; one CD4; two CD5; one wCD6; and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.