Crystallization and preliminary X-ray analysis of a vanadium-dependent peroxidase from Ascophyllum nodosum

Peroxidase from the brown alga Ascophyllum nodosum, a non‐heme vanadium‐dependent haloperoxidase, has been purified to homogeneity and crystallized from ammonium sulfate solutions in a form suitable for X‐ray diffraction analysis. The crystals have been grown by the vapour‐diffusion technique using...

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Published inActa crystallographica. Section D, Biological crystallography. Vol. 52; no. 4; pp. 864 - 865
Main Authors Weyand, M., Hecht, H.-J., Vilter, H., Schomburg, D.
Format Journal Article
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.07.1996
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Summary:Peroxidase from the brown alga Ascophyllum nodosum, a non‐heme vanadium‐dependent haloperoxidase, has been purified to homogeneity and crystallized from ammonium sulfate solutions in a form suitable for X‐ray diffraction analysis. The crystals have been grown by the vapour‐diffusion technique using the sitting‐drop method. X‐ray diffraction studies show that the crystals belong to the tetragonal space group P41212 or P43212 with a = b = 114.3 and c = 276.0 Å. The crystals diffract to at least 2.4 Å resolution.
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ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444996000959