Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium

• Published in: Acta crystallographica. Section D, Biological crystallography. Vol. 60; no. 2; pp. 342 - 343
• Main Authors: Nishimoto, Mamoru, Fushinobu, Shinya, Miyanaga, Akimasa, Wakagi, Takayoshi, Shoun, Hirofumi, Sakka, Kazuo, Ohmiya, Kunio, Nirasawa, Satoru, Kitaoka, Motomitsu, Hayashi, Kiyoshi
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.02.2004
• Subjects: Clostridium - enzymology; Crystallography, X-Ray; Detergents - pharmacology; Diffusion; Endo-1,4-beta Xylanases - chemistry; Escherichia coli - metabolism; Plasmids - chemistry; Plasmids - metabolism; Polyethylene Glycols - chemistry; Protein Conformation; Recombinant Proteins - chemistry; Temperature; xylanase B
• ISSN: 1399-0047; 0907-4449; 1399-0047
• DOI: 10.1107/S090744490302715X

Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting‐drop vapour‐diffusion method using 4 mg ml−1 purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non‐detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P212121, with unit‐cell parameters a = 64.76, b = 96.60, c = 138.44 Å. X‐ray diffraction data were collected to 1.80 Å resolution.