Purification, crystallization and Patterson search of haemoglobin IV from the armoured catfish Liposarcus anisitsi

• Published in: Acta crystallographica. Section D, Biological crystallography. Vol. 57; no. 9; pp. 1329 - 1331
• Main Authors: Delatorre, P., Smarra, A. L. S., Fadel, V., Canduri, F., Dellamano, M., Bonilla-Rodriguez, G. O., De Azevedo Jr, W. F.
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England Munksgaard International Publishers 01.09.2001
• Subjects: Animals; Catfishes; Crystallization; Crystallography, X-Ray; haemoglobin; Hemoglobins - chemistry; Hemoglobins - isolation & purification; Models, Molecular; Protein Conformation
• ISSN: 1399-0047; 0907-4449; 1399-0047
• DOI: 10.1107/S0907444901012057

Considerable interest is currently focused on fish haemoglobins in order to identify the structural basis for their diversity of functional behaviour. The armored catfish Liposarcus anisitsi presents accessorial air breathing through a modified stomach, which allows this species to survive in waters with low oxygen content. The analysis of its haemolysate has shown the presence of four main haemoglobins, with this work focusing on haemoglobin IV (LaHb‐IV). LaHb‐IV was crystallized and X‐ray diffraction data were collected to 2.4 Å resolution using a synchrotron‐radiation source. The crystal belongs to the orthorhombic space group P212121, with unit‐cell parameters a = 52.6 (1), b = 104.8 (2), c = 113.9 (2) Å; preliminary structural analysis revealed the presence of one tetramer in the asymmetric unit. The structure was determined using the standard molecular‐replacement technique.