Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis

Selenate reductase from Thauera selenatis was crystallized using ammonium sulfate as a precipitant. Crystals of selenate reductase belong to the space group C2, with unit‐cell parameters a = 116.9, b = 67.5, c = 186.7 Å, β = 90°. Native data to 2.1 Å resolution have been collected and a heavy‐atom d...

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Published inActa crystallographica. Section D, Biological crystallography. Vol. 58; no. 4; pp. 706 - 708
Main Authors Maher, Megan J., Macy, Joan M.
Format Journal Article
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England Munksgaard International Publishers 01.04.2002
Subjects
Crystallization
Crystallography, X-Ray
Oxidoreductases - chemistry
selenate reductase
Thauera - chemistry
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Summary:Selenate reductase from Thauera selenatis was crystallized using ammonium sulfate as a precipitant. Crystals of selenate reductase belong to the space group C2, with unit‐cell parameters a = 116.9, b = 67.5, c = 186.7 Å, β = 90°. Native data to 2.1 Å resolution have been collected and a heavy‐atom derivative has been identified following soaking of the crystals in a solution of trimethyl lead acetate.
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ark:/67375/WNG-XLBJMCJZ-T
ArticleID:AYDGR2226
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444902002640