Host Gene Regulation During Coxsackievirus B3 Infection in Mice: Assessment by Microarrays

• Published in: Circulation research Vol. 87; no. 4; pp. 328 - 334
• Main Authors: Taylor, Lydia A, Carthy, Christopher M, Yang, Decheng, Saad, Kareem, Wong, Donald, Schreiner, George, Stanton, Lawrence W, McManus, Bruce M
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD American Heart Association, Inc 18.08.2000; Lippincott; Lippincott Williams & Wilkins Ovid Technologies
• Subjects: Animals; Biological and medical sciences; Coxsackievirus Infections - genetics; Coxsackievirus Infections - immunology; Coxsackievirus Infections - metabolism; Energy Metabolism - physiology; Enterovirus - genetics; Experimental viral diseases and models; Gene Expression Regulation, Viral - immunology; Heart - virology; HeLa Cells; Humans; Immunocompetence; In Situ Hybridization; Infectious diseases; Medical sciences; Mice; Mice, Inbred A; Myocarditis - immunology; Myocarditis - metabolism; Myocarditis - virology; Oligonucleotide Array Sequence Analysis; RNA, Messenger - analysis; RNA, Viral - analysis; Time Factors; Viral diseases; Picornaviridae; Pathogenesis; Rodentia; Cardiovascular disease; Enterovirus; Host; Gene expression; Myocardial disease; Host virus relation; Infection; Virus; Myocarditis; Vertebrata; Regulation(control); Mammalia; Mouse; Heart disease; Animal; Coxsackievirus B3; Viral disease; Myocardium; Circulatory system
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/01.RES.87.4.328

Host genetic responses that characterize enteroviral myocarditis have not yet been determined. The injurious and inflammatory process in heart muscle may reflect host responses of benefit to the virus and ultimately result in congestive heart failure and dilated cardiomyopathy. On the other hand, host responses within the myocardium may secure the host against acute or protracted damage. To investigate the nature of modified gene expression in comparison with normal tissue, mRNA species were assessed in myocardium using cDNA microarray technology at days 3, 9, and 30 after infection. Of 7000 clones initially screened, 169 known genes had a level of expression significantly different at 1 or more postinfection time points as compared with baseline. The known regulated genes were sorted according to their functional groups and normalized expression patterns and, subsequently, interpreted in the context of viremic, inflammatory, and healing phases of the myocarditic process.