X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia

• Published in: Acta crystallographica. Section D, Biological crystallography. Vol. 59; no. 2; pp. 378 - 380
• Main Authors: Fujihashi, Masahiro, Peapus, Diane H., Nakajima, Eijiro, Yamada, Tsuyoshi, Saito, Jun-ichi, Kita, Akiko, Higuchi, Yoshiki, Sugawara, Yoko, Ando, Akikazu, Kamiya, Nobuo, Nagata, Yoshiho, Miki, Kunio
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England Munksgaard International Publishers 01.02.2003
• Subjects: Ascomycota - chemistry; Crystallization - methods; Crystallography, X-Ray - methods; fucose-specific lectins; Fungal Proteins - chemistry; Lectins - chemistry; Mercuric Chloride - chemistry; Static Electricity
• ISSN: 1399-0047; 0907-4449; 1399-0047
• DOI: 10.1107/S0907444902022175

A fucose‐specific lectin from Aleuria aurantia was crystallized in its native form and was also cocrystallized with HgCl2. Crystallization was performed using the sitting‐drop vapour‐diffusion method with ammonium sulfate as a precipitant. Both the Hg‐free native crystals and the Hg cocrystals belong to the hexagonal space group P6522. The unit‐cell parameters for the Hg‐free form are a = 84.0, c = 250.1 Å and for the Hg cocrystals are a = 83.9, c = 254.3 Å. Both forms of the crystals diffract X‐rays to 2.3 Å resolution and are suitable for high‐resolution crystal structure determination. Initial phasing was successfully performed by the MAD method using the Hg cocrystals and the electron density obtained was good enough for model building.