3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

Electrochemical spectroscopy enables rapid, sensitive, and label-free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen-printed electrodes (SPEs), a valuable, disposable alternati...

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Published inSensors (Basel, Switzerland) Vol. 20; no. 16; p. 4421
Main Authors Siller, Ina G, Preuss, John-Alexander, Urmann, Katharina, Hoffmann, Michael R, Scheper, Thomas, Bahnemann, Janina
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 07.08.2020
MDPI
Subjects
3-D printers
additive manufacturing
Antibodies
aptasensor
Automation
Bacteria
Biosensing Techniques
Biosensors
Design
E coli
Electrochemical Techniques
Electrodes
Escherichia coli
High resolution
impedimetric biosensor
Laboratories
Microfluidics
Printing, Three-Dimensional
Reagents
screen-printed electrodes
Three dimensional flow
Universal Serial Bus
United States > US
additive manufacturing
impedimetric biosensor
screen-printed electrodes
aptasensor
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Summary:Electrochemical spectroscopy enables rapid, sensitive, and label-free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen-printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio-)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high-resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor-made, experiment-specific systems. In this work, flow cells for SPE-based electrochemical (bio-)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamer-based impedimetric biosensor approach for the detection of ( ) Crooks strain as a proof of concept. Moreover, further developments towards a 3D-printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high-resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio-)sensor applications.
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Co-first author, these authors contributed equally to this work.
ISSN:1424-8220
1424-8220
DOI:10.3390/s20164421