Association of −308G/A TNF-α gene polymorphism and spontaneous preterm birth in Acehnese ethnic group, Indonesia: This polymorphism is not associated with preterm birth

• Published in: The Egyptian journal of medical human genetics Vol. 17; no. 1; pp. 33 - 40
• Main Authors: Andalas, Mohd, Hakimi, Mohammad, Nurdiati, Detty Siti, Astuti, Indwiani, Imran, Imran, Harapan, Harapan
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Elsevier B.V 01.01.2016; Egyptian Society of Human Genetics; SpringerOpen
• Subjects: Indonesia; Nucleotides; Premature labor; rs1800629; SNP; Spontaneous preterm birth; TNF-α; TNFA; rs1800629; TNFA; Spontaneous preterm birth; TNF-α; SNP; Indonesia
• ISSN: 1110-8630; 2090-2441
• DOI: 10.1016/j.ejmhg.2015.05.001

Single nucleotide polymorphism (SNP) within tumor necrosis factor alpha (TNF-α) gene promoter (−308G/A TNFA) is associated with higher gene expression. The role of this SNP as a risk factor for spontaneous preterm birth has been assessed in some regions and the findings were significantly different between race and ethnic groups. To provide the scientific evidence whether allele A within SNP −308G/A TNFA promoter is a risk factor for spontaneous preterm birth among Acehnese ethnic or not. In this case–control study, the genotypes of SNP −308G/A TNFA among 40 patients with spontaneous preterm birth and 40 patients with term birth were determined by real-time polymerase chain reaction (RT-PCR). The concentrations of TNF-α from blood were measured by enzyme-linked immunosorbent assay (ELISA). The differences in genotype distributions, dominant and recessive models, and allele frequencies between case and control groups were analyzed with Chi-squared test. Deviation of genotype frequencies from the Hardy–Weinberg equilibrium (HWE) was assessed by Fisher’s exact test. This study found that the concentration of TNF-α between preterm and control groups was not statistically different, 5.5±2.9mg/dL vs. 10.1±17.9mg/dL, p=0.112. The level of TNF-α had no strong association with either genotype distribution or allele frequency of SNP −308G/A TNFA. Furthermore, there was no association between mutant genotypes and spontaneous preterm birth (OR: 0.32; 95%CI: 0.08–1.33, p=0.096) and between mutant allele and spontaneous preterm birth (OR: 0.35; 95%CI: 0.09–1.37, p=0.105). SNP −308G/A TNFA is not associated with spontaneous preterm birth in Acehnese ethnic group.