Halomonas-PHB protects gnotobiotic Artemia against Vibrio and modifies Artemia gut microbiota in xenic culture conditions

• Published in: Journal of oceanology and limnology Vol. 41; no. 4; pp. 1292 - 1299
• Main Authors: Sui, Liying, Liu, Xiaocui, Pan, Namin, Liu, Xue, Gao, Meirong
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.07.2023; Springer Nature B.V; Key Laboratory of Marine Resource Chemistry and Food Technology(TUST),Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China; Asian Regional Artemia Reference Center,College of Marine and Environmental Sciences,Tianjin University of Science and Technology,Tianjin 300457,China
• Subjects: Accumulation; Acids; Algae; Aquaculture; Aquatic crustaceans; Artemia; Bacteria; Brine shrimp culture; Cell culture; Crustaceans; Cysts; Earth and Environmental Science; Earth Sciences; Feeding; Fish hatcheries; germ-free animals; Glucose; Gnotobiotic; Halomonas; Intestinal flora; Intestinal microflora; intestinal microorganisms; Isochrysis; Limnology; Live feeds; microalgae; microbial communities; Microbiota; Microorganisms; Next-generation sequencing; Oceanography; Pathogens; Pepsin; Phytoplankton; Polyculture (aquaculture); Prebiotics; prokaryotic cells; Salinity; Sequence analysis; Survival; Vibrio; Vibrio anguillarum; PHB; pathogen resistance; ploy-β-hydroxybutyrate; gut microbiota; survival; Halomonas-ploy-β-hydroxybutyrate(Halomonas-PHB); Artemia
• ISSN: 2096-5508; 2523-3521
• DOI: 10.1007/s00343-022-2175-1

The prokaryotic cell storage compound ploy-β-hydroxybutyrate (PHB) has been considered as prebiotics that can be applied in aquaculture. In this paper, the dietary effect of a PHB-accumulating Halomonas strain (HM·PHB) identified from our previous work were studied in Artemia under gnotobiotic and xenic culture conditions, in comparison of Halomonas without PHB accumulation (HM) and microalgae Isochrysis (ISO) feeding. Under gnotobiotic condition, both HM·PHB and HM served as sole food supporting Artemia survival. Although both HM·PHB and HM feeding had no significant difference on Artemia survival percentage ( P >0.05), HM·PHB significantly improved their resistance against Vibrio anguillarum challenge ( P <0.05). Mass Artemia culture were further performed in xenic condition. Compared to ISO, HM·PHB feeding protected Artemia against V. anguillarum challenge ( P <0.05), and HM·PHB and HM feeding resulted in increased T-AOC, pepsin, T-SOD and CAT activities ( P <0.05). High throughput sequencing analysis showed that HM·PHB and HM feeding resulted in a lower Artemia gut microbial diversity ( P <0.05), and modified the gut microbial community by remarkably reducing the Vibrio proportion. The outcome of the paper confirmed the beneficial effect of Halomonas -PHB in Artemia culture, which supports the use of Halomonas -PHB in the production of bio-secured live feed Artemia .