Effects of gestational diabetes on junctional adhesion molecules in human term placental vasculature

• Published in: Diabetologia Vol. 43; no. 9; pp. 1185 - 1196
• Main Authors: BABAWALE, M. O, LOVAT, S, MAYHEW, T. M, LAMMIMAN, M. J, JAMES, D. K, LEACH, L
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.09.2000; Springer Nature B.V
• Subjects: Angiogenesis; Antigens, CD; beta Catenin; Biological and medical sciences; Birth Weight; Blood vessels; Cadherins - analysis; Cell adhesion & migration; Cesarean Section; Cytoskeletal Proteins - analysis; Diabetes, Gestational - drug therapy; Diabetes, Gestational - physiopathology; Diseases of mother, fetus and pregnancy; Endothelium; Endothelium, Vascular - physiology; Endothelium, Vascular - physiopathology; Female; Gestational Age; Gestational diabetes; Glycated Hemoglobin A - analysis; Gynecology. Andrology. Obstetrics; Health sciences; Humans; Infant, Newborn; Insulin - therapeutic use; Medical sciences; Membrane Proteins - analysis; Occludin; Organ Size; Permeability; Phosphoproteins - analysis; Phosphorylation; Placenta; Placenta - anatomy & histology; Placenta - blood supply; Pregnancy; Pregnancy - physiology; Pregnancy. Fetus. Placenta; Proteins; Reference Values; Trans-Activators; Vascular endothelial growth factor; Zonula Occludens-1 Protein; United Kingdom > UK; Endocrinopathy; Human; Fetal membrane; Diabetes mellitus; Maternal diseases; Cadherin; Adhesion; Endothelium; Pregnancy; Placenta; Blood vessel; Circulatory system; Catenin
• ISSN: 0012-186X; 1432-0428
• DOI: 10.1007/s001250051511

The aim of this study was to investigate whether gestational diabetes mellitus, which occurs in the microvascular remodelling phase of placental development, causes alterations in surface expression of tight and adherens junctional molecules involved in endothelial barrier function and angiogenesis. Term placenta, delivered by elective Caesarian section, from normal pregnancy (n = 5) and those complicated by gestational diabetes (n = 5) were perfusion-fixed and analysed by indirect immunofluorescence and confocal scanning microscopy. Using systematic random sampling, the surface expression of endothelial junctional proteins and the relative incidences of immunostained vessels were compared between the two study groups. Total vessel lengths were measured by stereological techniques. The adherens junctional molecules, vascular-endothelial cadherin and beta-catenin, and the tight junctional molecules, occludin and zonula occludens-1 were localised to paracellular clefts in both study groups. The diabetic placentae showed pronounced reductions in the intensity of immunofluorescence and in the number of immuno-positive vessels. A corresponding statistically significant increase (from 19% to 56%) in the percentage of vessels showing junctional anti-phosphotyrosine immunoreactivity was found. The differences observed represented real changes in the absolute lengths of immunostained regions along the vessels. The stereological measurements failed to detect any statistically significant change in the combined length of fetal vessels in gestational diabetic placenta. Our results suggest that even short duration diabetic insult, alters the surface expression of placental junctional proteins. This alteration could be mediated by the tyrosine-phosphorylation pathway. The changes suggest impaired barrier function rather than accelerated vascular growth.