Characterisation of microsatellite DNA markers for Mirbelia bursarioides A.M.Monro & Crisp ms
A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape....
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Published in | Conservation genetics resources Vol. 6; no. 3; pp. 693 - 695 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer-Verlag
01.09.2014
Springer Netherlands Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. |
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Bibliography: | http://dx.doi.org/10.1007/s12686-014-0184-x |
ISSN: | 1877-7252 1877-7260 |
DOI: | 10.1007/s12686-014-0184-x |