Characterisation of microsatellite DNA markers for Mirbelia bursarioides A.M.Monro & Crisp ms

• Published in: Conservation genetics resources Vol. 6; no. 3; pp. 693 - 695
• Main Authors: Millar, Melissa A, Byrne, Margaret, Coates, David J, Roberts, J. Dale
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.09.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Alleles; Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Conservation Biology/Ecology; Ecology; Evolutionary Biology; Genetic diversity; Genetic markers; Genetic structure; genetic variation; genomic libraries; Heredity; landscapes; Life Sciences; loci; Microsatellite Letters; microsatellite repeats; Microsatellites; Mirbelia; null alleles; Plant Genetics and Genomics; Population genetics; shrubs; Western Australia; Ironstone; Western Australia; M13 tagged primer; Microsatellites; Granite
• ISSN: 1877-7252; 1877-7260
• DOI: 10.1007/s12686-014-0184-x

A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.