Changes of fatty acid profiles of hepatic subcellular fractions and plasma lipids in rats given iron-deficient diet and/or ethanol

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 34; no. 5; pp. 367 - 373_1
• Main Authors: Hirosue, T. (Kagawa Nutrition Coll., Sakado, Saitama (Japan)), Hosogai, Y
• Format: Journal Article
• Language: Japanese; English
• Published: Japanese Society for Food Hygiene and Safety 1993
• Subjects: BLOOD PLASMA; CARENCE MINERALE; DEFICIENCIA DE MINERALES; ETANOL; ETHANOL; fatty acid; FER; FOIE; HIERRO; HIGADO; IRON; iron-deficient anemia (rat); lipid; LIPID METABOLISM; LIVER; METABOLISME DES LIPIDES; METABOLISMO DE LIPIDOS; microsome; MINERAL DEFICIENCIES; mitochondria; plasma; PLASMA SANGUIN; PLASMA SANGUINEO; RAT; RATA; RATS
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.34.367

This investigation was undertaken to elucidate the effects of iron-deficiency and/or ethanol ingestion on lipid metabolism of female rat liver. Forty Wistar female rats (about 40g b. w.) were fed a normal diet (Fe: 40ppm) or an iron-deficient diet (Fe: 5ppm) for 8 weeks. Ethanol (10%) was simultaneously given to half of each group as drinking water for the last 4 weeks. Rats were killed, the liver was removed, and the lipid from the homogenate was extracted and separated into several fractions. The fatty acids were determined by GC. In rats fed the iron-deficient diet, the proportions of the following fatty acids with respect to the total fatty acid levels were increased: 22:6 in hepatic phospholipid, 18:2 and 18:3 in hepatic mitochondrial lipid, 18:2 in hepatic microsomal lipid, and 18:0 and 22:6 in plasma lipid, and conversely those of the following fatty acids were reduced: 16:1, 18:1 and 20:4 in hepatic phospholipid, and 16:1 and 18:1 in plasma lipid. In normal rats given ethanol, the proportions of the following fatty acids with respect to the total fatty acid levels were increased: 16:1 in phospholipid, 12:0, 14:0, 16:1, 18:1 and 18:3 in mitochondrial lipid, 12:0, 14:0 and 16:1 in microsomal lipid, and 16:1 in plasma lipid, while the proportions of 18:0, 20:4, 24:0 and 22:6 in mitochondrial lipid were reduced. In the iron-deficient animals given ethanol, the percentages with respect to the total fatty acid of 24:0 in phospholipid, of 14:0, 16:0 and 18:3 in mitochondrial lipid and of 14:0 in microsomal lipid were increased, but those of 18:0 and 22:6 in mitochondrial lipid were reduced. The data might suggest that iron-deficiency alleviates the change in the fatty acid patterns of rat liver caused by ethanol.