Effect of differentiation on platelet-activating factor metabolism in HL-60 cells

• Published in: Journal of cell science Vol. 100; no. 1; pp. 145 - 152
• Main Authors: STOLL, L. L, YERRAM, N. R, SPECTOR, A. A
• Format: Journal Article
• Language: English
• Published: Cambridge Company of Biologists 01.09.1991
• Subjects: Biological and medical sciences; Cell Differentiation; Cell physiology; Fundamental and applied biological sciences. Psychology; Glyceryl Ethers - metabolism; Humans; Molecular and cellular biology; Phenotype; Platelet Activating Factor - metabolism; platelet-activating factor; Responses to growth factors, tumor promotors, other factors; Tumor Cells, Cultured; Human; Protein kinase C; Binding capacity; Enzyme; Activator; Metabolite; Cell differentiation; Metabolism; Biological activity; Platelet activating factor; Growth factor
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.100.1.145

The formation and metabolism of 1-O-alkyl-2-acetyl-sn-glycerol (AAG), a protein kinase C (PKC) activator formed from platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3- phosphocholine; PAF), was studied in HL-60 cells to determine whether differentiation may influence this process. HL-60 cells differentiated to macrophages (HL-60/M phi) with a phorbol ester convert added [3H]PAF to AAG; 22% of the incorporated radioactivity is converted to AAG within 15s. By contrast, neither undifferentiated HL-60 cells (HL-60/U) nor HL-60 cells differentiated to granulocytes (HL-60/GN) with retinoic acid produce AAG from PAF. The HL-60/M phi rapidly convert radiolabeled AAG to 1-O-alkyl-sn-glycerol and, subsequently, to two other unidentified metabolites. However, some apparently unmodified AAG persists in the cell lipids for at least 6 h. The HL-60 subtypes which do not convert PAF to AAG can nevertheless catabolize AAG; HL-60/U and HL-60/GN produce alkylglycerol and the other AAG metabolites. These findings demonstrate that differentiation can alter the processing of PAF in a human leukocyte cell line. Furthermore, they suggest that PAF may produce at least some of its biological effects in macrophages by conversion to AAG.